Processes and techniques

The celares GmbH established many processes and techniques to manufacture bioconjugates as well as small-molecules and artificial polymers. A selection of available techniques is listed here.

Bioconjugates manufacturing:

• Activation of capped thiol-groups
• Reduction and re-folding (oxidation) of disulfide-bridged proteins and peptides
• Conjugation chemistries (selection)
  • Reductive amination (including detector and scrubber for HCN)
  • Activated esters or carbonates
  • Thiol reactive groups
  • Click chemistry
  • Enzymatic conjugation
• Chromatography
• Tangential-flow filtration / ultrafiltration
• Batch size may range from 100 mg to 100 g
• Sterile filtration of drug substance in UDAF

Chemical compound manufacturing:

• Anionic polymerization of single or multiple, non-gaseous monomers
• Activation of polymers
• Handling and characterization of sensitive polymers and copolymers
• Temperature controlled reactions down to –15 °C
• Reactions in inert atmosphere
• Batch size may range from 100 g to 5 kg
• Safety relevant reactions (e.g., handling of toxic or flammable gases or products)

Small-volume liquid handling

High throughput production of components for diagnostics or lab-on-a-chip applications:

• Automated liquid handling of small volumes
• Drying or freeze-drying of small reagent solutions
• Functional quality control